Rapid Detection of Porcine Circovirus Type 2 by TaqMan-based Real-time Polymerase Chain Reaction Assays
نویسندگان
چکیده
PCV2 is the primary causative agent of porcine circovirus-associated disease (PCVAD). In this study, a TaqMan-based real-time polymerase chain reaction (PCR) assay targeting ORF2 gene of PCV2 was developed and their sensitivities and specificities were investigated. The results indicated that the standard curve had a wide dynamic range (102-107copies/μL) with a linear correlation (R2) of 0.997 between the cycle threshold (Ct) value and template concentration. The real-time PCR assay is highly sensitive and able to detect 2.5×101 copies/μL of PCV2 DNA, as no cross-reaction was observed with other viruses. These data suggested that the real-time PCR assay developed in this study will be suitable for future surveillance and specific diagnosis of PCV2-infection.
منابع مشابه
Development of Isothermal Recombinase Polymerase Amplification Assay for Rapid Detection of Porcine Circovirus Type 2
Porcine circovirus virus type II (PCV2) is the etiology of postweaning multisystemic wasting syndrome (PMWS), porcine dermatitis, nephropathy syndrome (PDNS), and necrotizing pneumonia. Rapid diagnosis tool for detection of PCV2 plays an important role in the disease control and eradication program. Recombinase polymerase amplification (RPA) assays using a real-time fluorescent detection (PCV2 ...
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